Part:BBa_K3153002:Design

oprF-fMT

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 608
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 697
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Based on the predicted secondary structure and information found in the literature, we chose 188aa(Val) as potential fusion sites for displaying fMT

Source

oprF, the major outer membrane (OM) protein, comes from Pseudomonas aeruginosa. fMT, an arsenic-chelating metallothionein, comes from a marine alga, Fucus vesiculosus